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Laboratory diagnostics of Hepatitis B
JANŮ, Denisa
Hepatitis B is an inflammatory viral disease of the liver. The etiological agent is a DNA virus from the Hepadnaviridae family. It is transmitted through the blood and secretions of an infected person. The greatest danger of the disease lies in the transition to chronicity. Chronic infection carries a high risk of developing liver cirrhosis and hepatocellular carcinoma. Hepatitis B is widespread throughout the world. The most reliable prevention is vaccination and avoidance of risky behavior and contact with persons with risky behavior, especially drug users and promiscuous persons. The aim of this bachelor thesis is to get acquainted with the disease of viral hepatitis B and to describe the current possibilities of diagnostics of hepatitis B markers using the COBAS e601 device from ROCHE by electrochemiluminescent immunoassay and quantification of viral DNA on the COBAS 4800 from ROCHE by real time PCR and evaluation of results using both methods. The set of examined samples comes from the laboratory of infectious serology and virology of the company VIDIA-DIAGNOSTIKA. The results show that HBsAg is the most investigated marker of hepatitis B. Positive findings make up only 0.67%. The ratio of HBsAg positive men and women in 2019 was almost balanced. Most HBsAg positive patients were aged 30-40 years. I also examined the prevalence of anti-HBc Ig total antibodies. The positivity of anti-HBc Ig total was confirmed in 4.27% of examinations. The ratio of anti-HBc Ig total positive men and women in 2019 was also almost balanced and the most positive were at the age of 30-40 years.
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The Awareness of Hepatitis B, C, D and Possible Ways of its Transmission at Intravenous Drug Users in Pardubice Region
TICHÁ, Žaneta
This thesis deals with the knowledge regarding viral hepatitis B, C and D considering intravenous drug application. Viral hepatitides represent one of big medical problems around the world. Hepatitis B, C and D belong among the most serious infectious diseases blood and sexually transmitted. However, these are not the only possible ways of transmission. At viral hepatitides there exists a risk of infection not always due to their own fault. We are all exposed to this risk and therefore it is important to inform and raise awareness about viral hepatitides not only among intravenous drug users but also general population. All over the world 2 billion of people are estimated to suffer viral hepatitis B, over 180 million hepatitis C and the number of people infected with viral hepatitis D is estimated for 15 million. Viral hepatitides can cause acute illness as well as we must take into account asymptomatic form of this disease. The infected itself does not have to know about his/her disease for a long time, therefore all the stated numbers are estimations. The theoretical part deals with actual situation ? in the first part there is defined the term intravenous drug user and there are listed the most important risks of this way of drug application. Next part is divided into five subchapters. In the first three subchapters I deal with viral hepatitides B, C and D. These subchapters describe an occurrence, source of infection, ways of transmission and symptoms of individual diseases. The second last subchapter describes prevention of viral hepatitides and the last one presents a treatment of viral hepatitides. Practical part includes a research. The research sample created intravenous drug users attending Kontaktní centrum Laxus o. s. (Contact Centre) in Pardubice and a field program in the Pardubice region. For this research I chose the method of questioning, the questionnaire technique. The result data were processed and consequently entered into graphs and tables. The results were assessed and compared to an available literature within a discussion. There were defined two targets and six hypotheses within the research.
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